This biosafety manual represents the institutional practices and procedures for the safe use and handling of biological materials, recombinant dna and synthetic nucleic acids at stanford university. The information provided should aid you in practicing safe science when working with biological agents. Cdcnih biosafety in microbiological and biomedical laboratories bmbl 5th edition website ut biohazardous waste basics. Who laboratory manual for the examination and processing of human semen 5th ed. Footnote 5 it is a guidance document for canadian facilities where human and terrestrial animal pathogens are handled, that updates the guidelines originally published as part ii of the. A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for working with biological agents.
California occupational safety and health administration calosha. The canadian biosafety handbook cbh, 2 nd edition, 2016 is intended as a companion document to the canadian biosafety standard cbs, 2 nd edition, 2015. The biosafety manual must be available and accessible. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. We wish to emphasize that the 5th edition of the bmbl remains an advisory document recommending best practices for the safe conduct of work in biomedical and clinical laboratories from a biosafety perspective, and is not intended as a regulatory document though we recognize that it will be used that way by some. Activities that are specifically addressed are those involving. Who laboratory manual for the examination and processing of. We promote biological safety at the nih through a broad range of support services, consultation and. This biosafety manual has been developed to provide general policies and procedures when working with biological agents at the university of nevada, reno. The purpose of the institutional biosafety manual is to assist both personnel and management in complying with the objectives of the cdcnih biosafety in microbiological and biomedical laboratories bmbl, 5th edition december 2009, nih guidelines for research involving recombinant or synthetic nucleic acid molecules april 2016, the. A spill of any quantity involving an agent infectious to humans, plants, or animals occurs in a public area.
This edition also includes safety information from the 1997 who publication safety in healthcare laboratories 1. Biosafety in microbiological and biomedical laboratories bmbl 5th edition personnel should adhere to standard procedures associated with other respiratory pathogens, such as seasonal influenza and other human coronaviruses, when they transport specimens within a facility. Laboratory biosecurity and risk assessment biosafety level 3 ag laboratories agent summary statements for some agricultural pathogens biological toxins at last count, over two hundred of our scientific and professional colleagues have assisted in the preparation of the 5th edition through participation in technical. Who laboratory biosafety manual lbm revision update. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes. Its primary focus should be to provide pertinent information to help execute the operations of the lab in a safe and professional manner. Chapman university biosafety manual 3 while not a regulation, the bmbl has been revised several times and is commonly seen as the gold standard for biosafety. The princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. Centers for disease controls and prevention and national institutes of health.
The following prudent biosafety practices are recommended by the national academy of sciences in the publication biosafety in the laboratory and in part constitute basic good biosafety practices. Introduction this manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. Select one of the links below for more information. For this new edition, the manual has been extensively revised and expanded. For this new edition, therefore, the manual has been. Receive training in standard microbiological techniques. The bmbl outlines four biosafety levels, summarized below. Biological safety manual arkansas state university. The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with bsl2 agents.
A laboratory acquired infection is known or suspected, or b. Chapman university is using the bmbl fifth edition, 2009 as the basis for this biosafety manual. Although bmbl5 only recommends practices we wish to emphasize that the 5th edition of the bmbl remains an advisory document recommending best practices for the safe conduct of work in biomedical and clinical laboratories from a biosafety. This manual only addresses those hazards associated with biological materials. The cdcnih biosafety in microbiological and biomedical laboratories, 5th edition, the osha occupational exposure to bloodborne pathogens standard, and the nih guidelines involving research with recombinant dna molecules are most often referred to for the biomedical research that is conducted at du. All research and teaching conducted by faculty, staff, students, volunteers, and visitors at odu involving any of the materials listed below must be approved by the odu institutional biosafety committee ibc prior to initiation. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. Jan 24, 20 the requirements at each laboratory biosafety level can be found in the cdcnih publication biosafety in microbiological and biomedical laboratories, 5th edition. Guidelines for safe work practices in human and animal medical diagnostic laboratories cdc cdc prevention guidelines database archive. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials. We wish to emphasize that the 5th edition of the bmbl remains an advisory document recommending best practices for the safe conduct of work in biomedical and clinical laboratories from a biosafety perspective, and is not intended as a regulatory document though we. Biosafety in microbiological and biomedical laboratories 5, 5th edition, revised decemnber 2009 edition by centers for disease control and prevention u. For specialized projectsactivities, the principle investigators should follow recommendations from 1 biosafety in microbiological and biomedical laboratories, 5th edition, cdcnih, 2007. Laboratory biosafety level criteria bsl1, bsl2, bsl3, bsl4 to comment on this section of the bmbl 5th edition, enter your responses below in the leave a reply box.
Refer to section ii of this manual for a general outline of good practices at bsl1, bsl2, and bsl3. Biosafety manual office of environmental health and safety. Canadian biosafety handbook, second edition canada. Biosafety manual california institute of technology. Who laboratory biosafety manual lbm lbm has served the global biosafety community for more than 30 years with practical guidance on biosafety who manual 1st edition, 1983 risk group. The bmbl outlines the biosafety level system that we have largely based the ut biosafety program upon. Sample laboratory biosafety manual for microbiological activities. Although these practices may be considered common sense and overly simplistic by experienced. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. The cau biosafety plan addresses the general research and teaching activities currently conducted on the cau sites. Principles and practices is still the leading comprehensive biosafety textbook available and is a pageturner as well. Biosafety procedure manual old dominion university.
The biosafety manual is designed in accordance with nih guidelines for research involving recombinant or synthetic nucleic acid molecules and the biosafety in microbiological and biomedical laboratories bmbl, 5th edition to assure laboratory personnel that with proper precautions. The fifth edition includes new information on sperm preparation for clinical use or specialized assays and on cyropreservation, an expanded section on quality control in the semen analysis laboratory and evidencebased reference ranges and reference limits for various semen characteristics. Basic, containment and maximum containment bsl yet to be defined technology. Descriptions of biosafety levels, as well as assigned biosafety levels for specific organisms, are contained in the cdcnih document, biosafety in microbiological and biomedical laboratories bmbl 5th edition. We facilitate the implementation of biosafety practices for researchers in the laboratory. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. Biosafety in microbiological and biomedical laboratories bmbl 5th edition cdcnih world health organization laboratory biosafety manual third edition who external icon.
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